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After incubation (10 min, 39C), the plates were centrifuged (200 x g, 5 min, 20C, Universal 320, Hettich, Tuttlingen, Germany) and the medium was replaced with pre-warmed XF Seahorse medium supplemented with glucose, sodium pyruvate and glutamine (all purchased from Agilent, Santa Clara, California, USA)

Conclusions Validation parameters including linearity, precision, and accuracy are within the ranges indicated by the FDA 33

PDIA6 domains (a 0 , a and b) and mutants (a 0 A5, b-ND) were constructed using a PrimeSTAR mutagenesis basal kit (Takara Bio)

This could be lipedema, a chronic condition causing a buildup of fatty tissue, typically in the legs, calves, hips, and buttocks areas

This suggests that the medication helps maintain weight loss but does not permanently change underlying biological drivers of weight gain